SFC Purification Platform

State-of-the-art supercritical fluid chromatography for chiral and achiral separations.
Faster, greener, orthogonal to preparative HPLC, and more productive.
Fully integrated into every Oxeltis synthesis and purification workflow, from early-stage method development to preparative-scale compound delivery.

01 — WHAT MAKES US DIFFERENT

Supercritical fluid chromatography is not a peripheral analytical tool at Oxeltis, it is a core purification capability embedded directly into our medicinal chemistry and custom synthesis workflows. Our hybrid SFC was selected specifically for its dual analytical and preparative capabilities within a single integrated platform, enabling us to develop, refine, and scale separation methods with precision, from first-pass method scouting to gram-scale compound delivery without changing instrument or protocol.

SFC offers distinct advantages over traditional HPLC for pharmaceutical research, such as reduced organic solvent use, faster run times, superior chiral selectivity, and compatibility with thermally sensitive molecules. Making it the purification technology of choice for drug discovery programs where stereochemical purity, speed, and sustainability are critical. At Oxeltis, we leverage these benefits by running the entire separation workflow (analytical screening, method optimization, loading studies, and preparative purification) on a single hybrid platform, eliminating method transfer uncertainties. Our scientists collaborate closely with clients to design scalable, technically rigorous, and regulatory-compliant separation strategies.

Key differentiators

Hybrid analytical-preparative platform on a single system — no method transfer risk between analytical scouting and scale-up

Broad column library

covering both chiral stationary phases and achiral columns for diverse separation challenges

One method, every scale

From rapid analytical screening to milligrams for early SAR, and up to 25 g/day for in vivo and preclinical supply

96-vial autosampler

for high-throughput analytical screening of chiral resolution conditions and method parameters

CO₂ recycling capability

reduced solvent waste and operational costs for high-throughput or long-running projects

02 — CAPABILITIES

What our SFC platform can do for your project

Chiral Separations & Enantiomeric Resolution

Resolution of enantiomers and diastereomers using a broad library of chiral stationary phases. Method development from analytical scouting to preparative isolation, with enantiomeric excess (ee) determination and chiral purity confirmation for every batch delivered.

Achiral Purification of Complex Mixtures

High-selectivity achiral separations for small molecules, peptides, and heterocyclic intermediates where HPLC resolution is insufficient. Particularly effective for closely related structural analogs, regioisomers, and compounds with similar polarity profiles.

Method Development & Optimization

Systematic screening of mobile phase composition, modifier ratios, column type, gradient conditions, and flow rates. Software-driven mode switching enables rapid iteration from analytical to preparative conditions without manual reconfiguration, accelerating method lock-in for time-sensitive projects.

Loading Studies & Preparative Scale-Up

Systematic compound loading studies to identify the optimal balance between throughput and purity before committing to preparative runs. Seamless scale-up from analytical (mg) to preparative (gram) quantities using the same method parameters, preserving resolution and minimizing re-optimization time.

Complementary analytical capabilities

03 — APPLICATION

Extended Polar Surface Area (EPSA)

Our SFC platform also supports EPSA (Extended Polar Surface Area) measurements, a powerful analytical technique for assessing the polar surface area of large, conformationally complex molecules such as peptides, macrocycles, and other beyond-rule-of-5 (bRo5) compounds. Unlike calculated PSA values, EPSA is experimentally determined from SFC retention data, providing a direct, conformation-sensitive measurement that better reflects the molecule's actual membrane permeability potential. This is particularly valuable for programs exploring macrocyclic scaffolds, stapled peptides, or other modalities where classical physicochemical predictors lose accuracy.

See our dedicated page covering our full EPSA methodology and capabilities for more details.

Need expert SFC purification for your project?

Whether it's a challenging chiral separation, a complex mixture, or a time-sensitive preparative campaign. Share your requirements and we'll respond within 48 hours.